What is "clenbuterol"?

ClenbuterolIt is a general term for a class of animal drugs. Substances that can promote the growth of lean meat and inhibit the growth of animal fat can be called "clenbuterol". It is a very cheap drug and has a very good effect on reducing fat and increasing lean meat (LeanMass). In the country, commonly referred to as "clenbuterol" refers to clenbuterol, ractopamine, salbutamol.

 

 

The harm of clenbuterol to human beings!

Clenbuterol can increase the unit economic value of pigs a lot, but because it is easy to accumulate residues in animal liver and can enter the human body through the food chain, it is seriously harmful to human health. After eating pork containing clenbuterol, people will experience dizziness, nausea, trembling hands and feet, rapid heartbeat and even cardiac arrest leading to coma and death, especially for patients with arrhythmia, hypertension, glaucoma, diabetes and hyperthyroidism.

 

Emphasis on clenbuterol.

1. The Ministry of Agriculture of China issued a document in 1997 prohibiting the use of clenbuterol in feed and livestock production;

2. The Ministry of Commerce has banned the import and export of ractopamine and ractopamine hydrochloride since December 9, 2009.

On December 27, 3.2001, February 9, 2002 and April 9, 2002, the Ministry of Agriculture respectively issued documents prohibiting the use of β-agonist drugs as feed additives for food animals (Ministry of Agriculture Announcement No. 176, 193 and Regulation No. 1519).

4. Notice of the General Office of the Ministry of Agriculture and Rural Affairs on the issuance of the "Special Action Plan for Combating Illegal Activities in the Field of Pig Slaughter and Doing a Good Job in the Prevention and Control of African Swine Fever.

5. Notice of the General Office of the Ministry of Agriculture and Rural Affairs on the issuance of the 2019 slaughter link "clenbuterol" supervision and testing and risk monitoring program.

6. Notice of the General Office of the Ministry of Agriculture and Rural Affairs on the issuance of the Key Points of Animal Husbandry and Veterinary Work in 2020.

 

Clenbuterol detection method

Methods for detecting clenbuterol, ractopamine and salbutamol ("clenbuterol"): one is chromatographic analysis, mainly including thin layer chromatography (TLC), gas chromatography (GC), high performance liquid chromatography (HPLC), gas-mass online (GC/MS), liquid-mass online (HPLC/MS), etc. Due to complicated instruments and complicated processes, not suitable for on-site monitoring and sample screening. The other is an immunological method, such as enzyme-linked immunosorbent assay (ELISA), which has the disadvantage of long detection time and high cost, which is not conducive to the promotion of use in grass-roots units. Moreover, both methods require professional technicians to operate.

 

 

Colloidal gold series productsApplicationindirect competitive immunochromatographic assayIn principle, the target in the sample binds to the colloidal gold-labeled specific antibody during the lateral flow, and continues to flow forward and compete with the fixed antigen on the NC membrane detection line for the colloidal gold-labeled specific antibody. If the target content in the sample is higher than or equal to the detection line, the detection line (T line) does not develop color and the result is positive; otherwise, the detection line (T line) develops color and the result is negative.

 

Solution

Beijing Nano-Ace colloidal gold series products apply the principle of indirect competitive immunochromatography technology.

 

 

Reference Figure Description

Negative: C line and T line are color, regardless of color depth are judged as negative.

Positive: C line color, T line does not color, were judged positive.

Invalid: C line does not color, T line regardless of whether color, are invalid.

Supplementary note:

Negative: The sample contains no drug or the drug content is below the detection limit.

Positive: The amount of drug in the sample is higher than or equal to the detection limit.

 

Detection limit

Name	Detection limit ppb(ng/mL)	Sample
Clenbuterol	3	Urine (pig/cow/sheep)
Ractopamine	5
Salbutamol	5

 

Small knowledge-identification method

1. Look at the thickness of the subcutaneous fat layer of pork. In the purchase of pork subcutaneous fat is too thin, too soft pork do not buy. Under normal circumstances, clenbuterol pigs grow due to taking medicine, and their subcutaneous fat layer is obviously thinner, usually less than 1cm; normal pigs will have a layer of fat between the cortex and lean meat, and the fat is about 1cm-2cm, too little Be careful.

Look at the color of the pork. Under normal circumstances, pork containing clenbuterol is particularly bright red and bright. Therefore, the lean meat part is too red, and the meat quality may not be normal.

3. In addition, the pork can also be cut into two or three fingers wide. If the pork is soft and cannot be set on the case, it may contain clenbuterol.

4, if the fat and lean meat have obvious separation, and lean meat and fat between the yellow liquid outflow may contain clenbuterol.